Hotspot

atpA

LSC · 36738-38261 · 1524 bp

Back to Phyllostachys

Pi 0.000327
MarkerSeek score 40.20
Primer available yes
Label atpA

Feature Metadata

label_name
atpA
feature_type
gene
region
LSC
length_bp
1524
variable_sites
1
pi
0.000327
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA_p1 TTCCCCTTGCTTCCGAAAAA TCTTTCTCAACGATTCCTCTTCT 45.0 39.1 57.9 57.3 1610-1619 bp 1.0 49.6
atpA_p2 TTTCCCCTTGCTTCCGAAAA TCTTTCTCAACGATTCCTCTTCT 45.0 39.1 57.9 57.3 1611-1620 bp 1.0 49.6
atpA_p3 TTTTCCCCTTGCTTCCGAAA TCTTTCTCAACGATTCCTCTTCT 45.0 39.1 57.9 57.3 1612-1621 bp 1.0 49.6
atpA_p4 TTTTTCCCCTTGCTTCCGAA TCTTTCTCAACGATTCCTCTTCT 45.0 39.1 57.9 57.3 1613-1622 bp 1.0 49.6
atpA_p5 TTCCCCTTGCTTCCGAAAAA TCTCAACGATTCCTCTTCTACT 45.0 40.9 57.9 56.1 1606-1615 bp 1.0 47.9