Hotspot

rps15-ndhF

IRb · 104687-105136 · 450 bp

Back to Phyllostachys

Pi 0

MarkerSeek score 42.10

Primer available yes

Label rps15-ndhF

Feature Metadata

label_name: rps15-ndhF
feature_type: igs
region: IRb
length_bp: 450
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhF_p1	GTTTGCTGGCTTATTTGGCA	AAATATGTAGGGGGAGGGCG	45.0	55.0	57.5	58.9	601-621 bp	1.0	71.0
rps15-ndhF_p2	GTTTGCTGGCTTATTTGGCA	GTAGGGGGAGGGCGAATAT	45.0	57.9	57.5	57.9	595-615 bp	1.0	69.8
rps15-ndhF_p3	GTTTGCTGGCTTATTTGGCA	GTAGGGGGAGGGCGAATATC	45.0	60.0	57.5	59.1	595-615 bp	1.0	69.7
rps15-ndhF_p4	CGTTTGCTGGCTTATTTGGC	AAATATGTAGGGGGAGGGCG	50.0	55.0	58.7	58.9	602-622 bp	1.0	69.4
rps15-ndhF_p5	ACGTTTGCTGGCTTATTTGG	AAATATGTAGGGGGAGGGCG	45.0	55.0	57.3	58.9	603-623 bp	1.0	68.8