Hotspot

psaA-ycf3

LSC · 43786-44417 · 632 bp

Back to Phyllostachys

Pi 0

MarkerSeek score 42.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 632
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGTTCTTGAGAAATGGCCGG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	804-813 bp	1.0	77.0
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	732-741 bp	1.0	77.0
psaA-ycf3_p3	TGTTCTTGAGAAATGGCCGG	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.5	58.1	800-809 bp	1.0	76.7
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.5	58.1	728-737 bp	1.0	76.7
psaA-ycf3_p5	GGTTTGGCCCATTCCTCAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.7	58.0	785-794 bp	1.0	75.6