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psbM-petN

LSC · 18665-19431 · 767 bp

Back to Phyllostachys

Pi 0.0022

MarkerSeek score 46.70

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 5
pi: 0.0022
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGGAAGTCAATATTCTCGCA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	54.5	58.2	939-954 bp	1.0	52.6
psbM-petN_p2	TGGAAGTCAATATTCTCGCA	GTAGTATGGGGGAGGAGTGG	40.0	60.0	54.5	58.3	883-898 bp	1.0	51.7
psbM-petN_p3	TGGAAGTCAATATTCTCGCA	TAGTATGGGGGAGGAGTGGA	40.0	55.0	54.5	58.4	882-897 bp	1.0	50.9
psbM-petN_p4	TGCTACTGCACTGTTTATTCT	TAGTAAGTCTCGCTTGGGCT	38.1	50.0	55.2	58.2	914-929 bp	1.0	50.7
psbM-petN_p5	TGCTACTGCACTGTTTATTCT	GTAGTATGGGGGAGGAGTGG	38.1	60.0	55.2	58.3	858-873 bp	1.0	49.8