Hotspot

psbZ-trnG-UCC

LSC · 12664-13606 · 943 bp

Back to Phyllostachys

Pi 0.000528
MarkerSeek score 40.40
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
943
variable_sites
1
pi
0.000528
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 TTGCTTCTCCTGATGGTTGG AGCCTTCCAAGCTAACGATG 50.0 50.0 57.8 58.0 1097 bp 1.0 78.2
psbZ-trnG-UCC_p2 TTGCTTCTCCTGATGGTTGG CCTAGCCTTCCAAGCTAACG 50.0 55.0 57.8 58.1 1100 bp 1.0 78.0
psbZ-trnG-UCC_p3 GCTTCTCCTGATGGTTGGTC AGCCTTCCAAGCTAACGATG 55.0 50.0 58.3 58.0 1095 bp 1.0 77.8
psbZ-trnG-UCC_p4 GCTTCTCCTGATGGTTGGTC CCTAGCCTTCCAAGCTAACG 55.0 55.0 58.3 58.1 1098 bp 1.0 77.6
psbZ-trnG-UCC_p5 TTGCTTCTCCTGATGGTTGG CCAACGTCGACTATAACCCC 50.0 55.0 57.8 57.8 1118 bp 1.0 76.7