Hotspot

rps16

LSC · 5558-6708 · 1151 bp

Back to Phyllanthus

Pi 0.0578

MarkerSeek score 82.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1151
variable_sites: 163
pi: 0.0578
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGTCCCGACAAAAAGAATCCA	CTGGATTACTATGTACCCGCG	42.9	52.4	57.5	58.1	1524-1540 bp	0.333333	35.6
rps16_p2	AGTCCCGACAAAAAGAATCCA	TGGATTACTATGTACCCGCG	42.9	50.0	57.5	56.9	1523-1539 bp	0.333333	35.3
rps16_p3	GAGAGAGTCCCGACAAAAAGA	CTGGATTACTATGTACCCGCG	47.6	52.4	57.3	58.1	1529-1545 bp	0.333333	34.3
rps16_p4	AGAGAGAGTCCCGACAAAAAG	CTGGATTACTATGTACCCGCG	47.6	52.4	57.3	58.1	1530-1546 bp	0.333333	34.2
rps16_p5	GAGAGAGTCCCGACAAAAAGA	TGGATTACTATGTACCCGCG	47.6	50.0	57.3	56.9	1528-1544 bp	0.333333	34.0