Hotspot

rpl22

LSC · 85273-85698 · 426 bp

Back to Phyllanthus

Pi 0.0814

MarkerSeek score 75.10

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 426
variable_sites: 85
pi: 0.0814
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TTGTCCCATAATCCCCCACT	TCCCGGGCATCTACCATTAT	50.0	50.0	58.0	58.0	793-861 bp	1.0	91.6
rpl22_p2	TTGTCCCATAATCCCCCACT	AATTCACCGTGACACGTTCA	50.0	45.0	58.0	58.1	903-971 bp	1.0	91.1
rpl22_p3	TTGTCCCATAATCCCCCACT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1082-1150 bp	0.833333	83.9
rpl22_p4	TTGTCCCATAATCCCCCACT	GACAAGTAGGGAATGCTGGG	50.0	55.0	58.0	58.0	1217-1285 bp	0.833333	83.6
rpl22_p5	TTGTCCCATAATCCCCCACT	GGACAAGTAGGGAATGCTGG	50.0	55.0	58.0	58.0	1218-1286 bp	0.833333	83.6