Hotspot

ndhF-rpl32

SSC · 113668-114627 · 960 bp

Back to Phyllanthus

Pi 0.0919
MarkerSeek score 78.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
960
variable_sites
186
pi
0.0919
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.80
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TCCATGAATATTGATATGTATGTTCCA TACGTTTCTTTGGAACTGCC 29.6 45.0 56.7 56.3 640-1010 bp 1.0 52.0
ndhF-rpl32_p2 CCATGAATATTGATATGTATGTTCCA TACGTTTCTTTGGAACTGCC 30.8 45.0 55.5 56.3 639-1009 bp 1.0 52.0
ndhF-rpl32_p3 TCCATGAATATTGATATGTATGTTCC TACGTTTCTTTGGAACTGCC 30.8 45.0 55.3 56.3 640-1010 bp 1.0 52.0
ndhF-rpl32_p4 CCATGAATATTGATATGTATGTTCC TACGTTTCTTTGGAACTGCC 32.0 45.0 54.0 56.3 639-1009 bp 1.0 52.0
ndhF-rpl32_p5 CCATGAATATTGATATGTATGTTCCAT TACGTTTCTTTGGAACTGCC 29.6 45.0 56.0 56.3 639-1009 bp 1.0 52.0