Hotspot

rbcL-accD

LSC · 57886-58635 · 750 bp

Back to Phyllanthus

Pi 0.0441

MarkerSeek score 82.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 85
pi: 0.0441
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATGAGGGACGTGATCTTGC	CCGAAATTCCGAATGTCCCT	50.0	50.0	58.0	58.0	1112-1167 bp	0.833333	81.4
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	TCGCTACCGTTCGTACTAGT	55.0	50.0	58.0	58.0	1461-1527 bp	0.666667	74.9
rbcL-accD_p3	CTTAACAGGGGGATTCACCG	TCGCTACCGTTCGTACTAGT	55.0	50.0	58.0	58.0	1993-2059 bp	0.666667	73.3
rbcL-accD_p4	CGCTGGTACCGTAGTAGGTA	TCGCTACCGTTCGTACTAGT	55.0	50.0	58.0	58.0	1819-1885 bp	0.5	64.6
rbcL-accD_p5	AATGAGGGACGTGATCTTGC	TCGCTACCGTTCGTACTAGT	50.0	50.0	58.0	58.0	1520-1572 bp	0.5	64.3