Hotspot

trnD-GUC-trnY-GUA

LSC · 30765-31191 · 427 bp

Back to Phyllanthus

Pi 0.0575
MarkerSeek score 85.40
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
427
variable_sites
65
pi
0.0575
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 509-529 bp 1.0 84.3
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 50.0 50.0 58.5 57.6 508-528 bp 1.0 84.1
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA GATTCTTCCTGGGTCGATGC 50.0 55.0 58.5 58.4 542-562 bp 1.0 83.6
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA CGGACTGTAAATTCGTTGGC 50.0 50.0 58.5 57.5 504-524 bp 1.0 83.0
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA TGGGGACGGACTGTAAATTC 50.0 50.0 58.5 57.2 510-530 bp 1.0 80.9