Hotspot

atpH-atpI

LSC · 14011-15130 · 1120 bp

Back to Phyllanthus

Pi 0.0457
MarkerSeek score 83.00
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1120
variable_sites
137
pi
0.0457
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TATGGTCCATGGGTTCCCTT 45.0 50.0 58.0 58.0 1603-1635 bp 1.0 91.1
atpH-atpI_p2 CAGTGACCCAAGGAAACGAA TATGGTCCATGGGTTCCCTT 50.0 50.0 58.0 58.0 2233-2364 bp 0.833333 83.3
atpH-atpI_p3 CCTCAAATCAGTCCTTCCCG TATGGTCCATGGGTTCCCTT 55.0 50.0 58.0 58.0 1866-1956 bp 0.833333 83.2
atpH-atpI_p4 CAGTGACCCAAGGAAACGAA TCGGTCATTGTAGCTGTTCG 50.0 50.0 58.0 58.0 2347-2478 bp 0.833333 83.1
atpH-atpI_p5 CCTCAAATCAGTCCTTCCCG TCGGTCATTGTAGCTGTTCG 55.0 50.0 58.0 58.0 1980-2070 bp 0.833333 83.0