Hotspot

trnS-GCU-trnR-UCU

LSC · 9067-10930 · 1864 bp

Back to Phyllanthus

Pi 0.0664

MarkerSeek score 83.10

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1864
variable_sites: 290
pi: 0.0664
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	AATCCGACGCTTTAGTCCAC	AGGAACAGCTGGAACGTTTT	50.0	45.0	58.0	57.9	2228-2332 bp	1.0	91.5
trnS-GCU-trnR-UCU_p2	ATCTAATGATCCGGGGCGTA	AGGAACAGCTGGAACGTTTT	50.0	45.0	58.1	57.9	2463-2570 bp	1.0	91.0
trnS-GCU-trnR-UCU_p3	TATCTAATGATCCGGGGCGT	AGGAACAGCTGGAACGTTTT	50.0	45.0	58.1	57.9	2464-2571 bp	1.0	91.0
trnS-GCU-trnR-UCU_p4	CAATCCGACGCTTTAGTCCA	AGGAACAGCTGGAACGTTTT	50.0	45.0	58.0	57.9	2229-2333 bp	0.833333	84.9
trnS-GCU-trnR-UCU_p5	CCATTCCCAGAAACCGCTTA	AGGAACAGCTGGAACGTTTT	50.0	45.0	57.9	57.9	2667-2728 bp	0.5	66.5