Hotspot

trnK-UUU-rps16

LSC · 4340-5557 · 1218 bp

Back to Phyllanthus

Pi 0.0663
MarkerSeek score 84.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1218
variable_sites
197
pi
0.0663
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAGTCGCACTTAAAAGCCGA CCCAATGAGCCGTTTATCGA 45.0 50.0 58.1 58.1 1360-1497 bp 1.0 90.6
trnK-UUU-rps16_p2 AAGTCGCACTTAAAAGCCGA TCCCAATGAGCCGTTTATCG 45.0 50.0 58.1 58.1 1361-1498 bp 1.0 90.6
trnK-UUU-rps16_p3 AAGTCGCACTTAAAAGCCGA GCCGTACGAGGAGAAAACTT 45.0 50.0 58.1 57.9 1421-1559 bp 1.0 90.4
trnK-UUU-rps16_p4 TACACACTCATCTCCCCCTC CCCAATGAGCCGTTTATCGA 55.0 50.0 57.8 58.1 1812-1946 bp 0.666667 74.7
trnK-UUU-rps16_p5 TACACACTCATCTCCCCCTC TCCCAATGAGCCGTTTATCG 55.0 50.0 57.8 58.1 1813-1947 bp 0.666667 74.7