Hotspot

matK-trnK-UUU

LSC · 3557-4302 · 746 bp

Back to Phyllanthus

Pi 0.0461

MarkerSeek score 83.30

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 87
pi: 0.0461
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GCTCAAGAAGGACCCGAAAA	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.1	58.2	1482-1509 bp	1.0	88.2
matK-trnK-UUU_p2	GCTCAAGAAGGACCCGAAAA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.1	58.2	1481-1508 bp	1.0	88.2
matK-trnK-UUU_p3	GATTCGCTCAAGAAGGACCC	AACGGTAGAGTACTCGGCTT	55.0	50.0	58.3	58.2	1487-1514 bp	1.0	86.3
matK-trnK-UUU_p4	GATTCGCTCAAGAAGGACCC	ACGGTAGAGTACTCGGCTTT	55.0	50.0	58.3	58.2	1486-1513 bp	1.0	86.3
matK-trnK-UUU_p5	GCTCAAGAAGGACCCGAAAA	TTGGGTTGCTAACTCAACGG	50.0	50.0	58.1	58.4	1500-1524 bp	0.833333	79.4