Hotspot

ycf1

SSC · 125238-130745 · 5508 bp

Back to Phyllagathis

Pi 0.0143

MarkerSeek score 72.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5508
variable_sites: 865
pi: 0.0143
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTGTGTAGTGATTCTCGGACT	CCTTTTGGTTCTCCCCGAAA	45.5	50.0	57.8	58.0	556-679 bp	0.886364	70.0
ycf1_p2	GTTGTGTAGTGATTCTCGGACT	ACGGTATTCCCAACAATCCG	45.5	50.0	57.8	58.0	1247-1382 bp	0.886364	69.8
ycf1_p3	AGTTGTGTAGTGATTCTCGGAC	CCTTTTGGTTCTCCCCGAAA	45.5	50.0	57.8	58.0	557-680 bp	0.863636	69.1
ycf1_p4	AGTTGTGTAGTGATTCTCGGAC	ACGGTATTCCCAACAATCCG	45.5	50.0	57.8	58.0	1248-1383 bp	0.840909	68.0
ycf1_p5	TGAATGGGAATGGTAAGGGATG	CCTTTTGGTTCTCCCCGAAA	45.5	50.0	57.8	58.0	221-344 bp	0.818182	67.2