Hotspot

psbA

LSC · 312-1373 · 1062 bp

Back to Phyllagathis

Pi 0.00751

MarkerSeek score 58.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 91
pi: 0.00751
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCAAAAACTCGTCTAAATTCAGACT	AGAATTCGTGTGCTTGGGAG	32.0	50.0	57.0	58.2	1151-1159 bp	0.886364	42.5
psbA_p2	TCAAAAACTCGTCTAAATTCAGACT	AGAGAATTCGTGTGCTTGGG	32.0	50.0	57.0	58.2	1153-1161 bp	0.886364	42.5
psbA_p3	TCAAAAACTCGTCTAAATTCAGACT	GAGAATTCGTGTGCTTGGGA	32.0	50.0	57.0	58.2	1152-1160 bp	0.886364	42.5
psbA_p4	TCAAAAACTCGTCTAAATTCAGACT	TGGGAGTCCCTGATGATGAA	32.0	50.0	57.0	57.7	1137-1145 bp	0.886364	42.4
psbA_p5	TCAAAAACTCGTCTAAATTCAGACT	TTGGGAGTCCCTGATGATGA	32.0	50.0	57.0	57.7	1138-1146 bp	0.886364	42.4