Hotspot

atpB-rbcL

LSC · 54979-55800 · 822 bp

Back to Phyllagathis

Pi 0.00994

MarkerSeek score 81.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 822
variable_sites: 109
pi: 0.00994
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTTCCCGGGGGTAAAGGATA	GCTGCCAAGGTATCAGTAGG	55.0	55.0	58.2	57.8	1030-1086 bp	1.0	88.5
atpB-rbcL_p2	ACTCCAGGAACCGAAGTAGT	GCTGCCAAGGTATCAGTAGG	50.0	55.0	57.7	57.8	948-1004 bp	1.0	87.6
atpB-rbcL_p3	TGGACCAATGATTTGAGCGA	GCTGCCAAGGTATCAGTAGG	45.0	55.0	57.5	57.8	1000-1056 bp	1.0	86.1
atpB-rbcL_p4	TTCCCGGGGGTAAAGGATAC	GCTGCCAAGGTATCAGTAGG	55.0	55.0	58.5	57.8	1029-1085 bp	1.0	86.1
atpB-rbcL_p5	GGAAACTCCAGGAACCGAAG	GCTGCCAAGGTATCAGTAGG	55.0	55.0	58.2	57.8	952-1008 bp	0.931818	85.8