Hotspot

ndhC-trnV-UAC

LSC · 51263-51927 · 665 bp

Back to Phyllagathis

Pi 0.0244

MarkerSeek score 72.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 157
pi: 0.0244
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AATGCCAAAAGTGGAATACCA	GGTTCGAATCCGTATAGCCC	38.1	55.0	56.0	57.9	465-957 bp	0.886364	62.9
ndhC-trnV-UAC_p2	GAAATGCCAAAAGTGGAATACCA	GGTTCGAATCCGTATAGCCC	39.1	55.0	57.9	57.9	467-959 bp	0.886364	61.7
ndhC-trnV-UAC_p3	AATGCCAAAAGTGGAATACCA	CGGTTCGAATCCGTATAGCC	38.1	55.0	56.0	58.3	466-958 bp	0.886364	61.1
ndhC-trnV-UAC_p4	AAATGCCAAAAGTGGAATACCA	GGTTCGAATCCGTATAGCCC	36.4	55.0	56.8	57.9	466-958 bp	0.886364	60.9
ndhC-trnV-UAC_p5	GAAATGCCAAAAGTGGAATACCA	CGGTTCGAATCCGTATAGCC	39.1	55.0	57.9	58.3	468-960 bp	0.886364	59.8