Hotspot

rpoB-trnC-GCA

LSC · 27081-28499 · 1419 bp

Back to Phyllagathis

Pi 0.0196

MarkerSeek score 78.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1419
variable_sites: 309
pi: 0.0196
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GACATTCCCTCACTTCCATCC	CCGGATTTGAACTGGGGAAA	52.4	50.0	58.1	58.1	1352-1816 bp	0.954545	80.9
rpoB-trnC-GCA_p2	TCACTTCCATCCACCAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.0	58.1	1343-1807 bp	0.931818	80.6
rpoB-trnC-GCA_p3	TTCCCTCACTTCCATCCACC	CCGGATTTGAACTGGGGAAA	55.0	50.0	59.0	58.1	1348-1617 bp	0.909091	79.6
rpoB-trnC-GCA_p4	CATTCCCTCACTTCCATCCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1350-1619 bp	0.886364	77.9
rpoB-trnC-GCA_p5	ATTCCCTCACTTCCATCCAC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1349-1618 bp	0.886364	77.9