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atpH-atpI

LSC · 13830-14780 · 951 bp

Back to Phyllagathis

Pi 0.0158

MarkerSeek score 82.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 951
variable_sites: 163
pi: 0.0158
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GAAGCAAGTCCGACAGCTAA	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.9	57.9	626-1241 bp	1.0	90.3
atpH-atpI_p2	GAAGCAAGTCCGACAGCTAA	TTTTTGCAACTTTAGCCGCC	50.0	45.0	57.9	58.1	638-1253 bp	1.0	90.2
atpH-atpI_p3	AACAGAAGCGGCAGAAATCA	TAGCCGCCGCTTATATAGGA	45.0	50.0	58.1	57.9	594-1209 bp	1.0	90.1
atpH-atpI_p4	AACAGAAGCGGCAGAAATCA	TTTTTGCAACTTTAGCCGCC	45.0	45.0	58.1	58.1	606-1221 bp	1.0	90.0
atpH-atpI_p5	CAGCAGCAATAACAGAAGCG	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.8	57.9	604-1219 bp	1.0	89.6