Hotspot

trnR-UCU-atpA

LSC · 9849-10157 · 309 bp

Back to Phyllagathis

Pi 0.0482

MarkerSeek score 85.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 309
variable_sites: 112
pi: 0.0482
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	CCAAACCCGAAGCCAAAAAG	CATTCACCGAGGAAGCAGAA	50.0	50.0	58.1	57.9	450-522 bp	0.840909	83.8
trnR-UCU-atpA_p2	CCAAACCCGAAGCCAAAAAG	AAGACATTCACCGAGGAAGC	50.0	50.0	58.1	57.9	454-526 bp	0.818182	82.8
trnR-UCU-atpA_p3	CCAAACCCGAAGCCAAAAAG	TTCACCGAGGAAGCAGAATC	50.0	50.0	58.1	57.6	448-520 bp	0.840909	81.5
trnR-UCU-atpA_p4	CCGAAGCCAAAAAGCAAGAA	CATTCACCGAGGAAGCAGAA	45.0	50.0	57.5	57.9	444-516 bp	0.840909	80.8
trnR-UCU-atpA_p5	CCAAACCCGAAGCCAAAAAG	CCGAGGAAGCAGAATCCATT	50.0	50.0	58.1	57.7	444-516 bp	0.795455	80.0