Hotspot

trnS-GCU-trnR-UCU

LSC · 7969-9776 · 1808 bp

Back to Phyllagathis

Pi 0.0237
MarkerSeek score 80.50
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1808
variable_sites
402
pi
0.0237
unique_haplotype_count
44
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 CAATCCGACGCTTTAGTCCA AGAACTTGCGTCCAATAGGA 50.0 45.0 58.0 56.6 1882-1986 bp 1.0 80.4
trnS-GCU-trnR-UCU_p2 AATCCGACGCTTTAGTCCAC AGAACTTGCGTCCAATAGGA 50.0 45.0 58.0 56.6 1881-1985 bp 1.0 80.4
trnS-GCU-trnR-UCU_p3 CAATCCGACGCTTTAGTCCA ACATAGAAGAACTTGCGTCCA 50.0 42.9 58.0 57.3 1889-1993 bp 1.0 78.5
trnS-GCU-trnR-UCU_p4 AATCCGACGCTTTAGTCCAC ACATAGAAGAACTTGCGTCCA 50.0 42.9 58.0 57.3 1888-1992 bp 1.0 78.5
trnS-GCU-trnR-UCU_p5 CAATCCGACGCTTTAGTCCA AAGAACTTGCGTCCAATAGGA 50.0 42.9 58.0 57.3 1883-1987 bp 1.0 78.4