Hotspot

ycf1

IRb · 117814-118911 · 1098 bp

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Pi 0.0273

MarkerSeek score 68.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1098
variable_sites: 70
pi: 0.0273
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCGGAACTTCTTCGTAGTG	GGGTTGGTGTTGCAAGTTTC	55.0	50.0	58.0	58.1	1226-1565 bp	0.6	71.0
ycf1_p2	CCGGAACTTCTTCGTAGTGG	GGGTTGGTGTTGCAAGTTTC	55.0	50.0	58.0	58.1	1225-1564 bp	0.6	71.0
ycf1_p3	GCTCCGTTCCATGTCTCATT	GGGTTGGTGTTGCAAGTTTC	50.0	50.0	58.0	58.1	1206-1545 bp	0.6	70.9
ycf1_p4	GAGCTACTGAGGAACAACGG	GGGTTGGTGTTGCAAGTTTC	55.0	50.0	58.0	58.1	1321-1660 bp	0.6	70.8
ycf1_p5	GAAACAAAGACAGCGAACGG	GGGTTGGTGTTGCAAGTTTC	50.0	50.0	58.0	58.1	1478-1817 bp	0.6	70.4