Hotspot

rpl16

LSC · 88862-90566 · 1705 bp

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Pi 0.00729

MarkerSeek score 67.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1705
variable_sites: 28
pi: 0.00729
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCATCCTCCATACACCGTTT	TCGATTGACCATTTGTTATAATTGCT	45.0	30.8	56.2	58.0	1723-1755 bp	1.0	45.3
rpl16_p2	TTCATCCTCCATACACCGTT	TCGATTGACCATTTGTTATAATTGCT	45.0	30.8	56.2	58.0	1724-1756 bp	1.0	45.3
rpl16_p3	TTTCATCCTCCATACACCGT	TCGATTGACCATTTGTTATAATTGCT	45.0	30.8	56.2	58.0	1725-1757 bp	1.0	45.3
rpl16_p4	AGGATTGCAAAGGATGAAGGA	TCGATTGACCATTTGTTATAATTGCT	42.9	30.8	57.3	58.0	1790-1822 bp	1.0	45.2
rpl16_p5	AGGATTGCAAAGGATGAAGGA	AGGTTGAATAAAAATTCGATTGACCA	42.9	30.8	57.3	58.1	1805-1837 bp	1.0	45.2