Pi
0.00797
MarkerSeek score
50.80
Primer available
yes
Label
clpP
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| clpP_p1 | GATTTACACGAAATCCCCAGT | AAGCCATTGTTACGTTTCCA | 42.9 | 40.0 | 56.0 | 55.9 | 2184-2195 bp | 1.0 | 43.0 |
| clpP_p2 | GATTTACACGAAATCCCCAGT | AAAGCCATTGTTACGTTTCCA | 42.9 | 38.1 | 56.0 | 56.6 | 2185-2196 bp | 1.0 | 43.0 |
| clpP_p3 | GATTTACACGAAATCCCCAGT | AGCCATTGTTACGTTTCCAT | 42.9 | 40.0 | 56.0 | 55.6 | 2183-2194 bp | 1.0 | 43.0 |
| clpP_p4 | GATTTACACGAAATCCCCAGT | GGGGATAAAGACGATAAAGCCA | 42.9 | 45.5 | 56.0 | 57.9 | 2200-2211 bp | 1.0 | 42.9 |
| clpP_p5 | GATTTACACGAAATCCCCAGT | AGGGGATAAAGACGATAAAGCC | 42.9 | 45.5 | 56.0 | 57.6 | 2201-2212 bp | 1.0 | 42.9 |