Hotspot

ycf2-trnI-CAU

IRa · 163885-163950 · 66 bp

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Pi 0.0439

MarkerSeek score 65.50

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 66
variable_sites: 6
pi: 0.0439
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	CTCAATTCGACGATCCAGGA	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.1	58.0	139-363 bp	1.0	75.6
ycf2-trnI-CAU_p2	TCTCAATTCGACGATCCAGG	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.1	58.0	140-364 bp	1.0	75.6
ycf2-trnI-CAU_p3	CTCAATTCGACGATCCAGGA	AGTTGGGCGCTTTAACCATT	50.0	45.0	57.1	58.4	140-364 bp	1.0	72.7
ycf2-trnI-CAU_p4	TCTCAATTCGACGATCCAGG	AGTTGGGCGCTTTAACCATT	50.0	45.0	57.1	58.4	141-365 bp	1.0	72.6
ycf2-trnI-CAU_p5	CTCAATTCGACGATCCAGGA	TTGGGCGCTTTAACCATTCA	50.0	45.0	57.1	58.4	138-362 bp	1.0	72.6