Hotspot

psaC-ndhE

SSC · 126833-128537 · 1705 bp

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Pi 0.00844

MarkerSeek score 50.00

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 1705
variable_sites: 27
pi: 0.00844
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ATACATCTGTAGGGCAGGCT	CCAGATCATCGAACAGGACC	50.0	55.0	57.9	57.8	1041-2529 bp	1.0	82.5
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	CCAGATCATCGAACAGGACC	50.0	55.0	58.1	57.8	1067-2555 bp	1.0	82.3
psaC-ndhE_p3	AAAAATCTGTTGGGCAAGCG	CCAGATCATCGAACAGGACC	45.0	55.0	57.8	57.8	1152-2640 bp	1.0	82.0
psaC-ndhE_p4	AGTCCATCGATTCCCAGTCT	CCAGATCATCGAACAGGACC	50.0	55.0	57.8	57.8	1599-2995 bp	0.8	73.2
psaC-ndhE_p5	ATCGATTCCCAGTCTCCAGT	CCAGATCATCGAACAGGACC	50.0	55.0	57.8	57.8	1594-2990 bp	0.8	73.2