Hotspot

ndhF-rpl32

SSC · 121240-122499 · 1260 bp

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Pi 0.0117

MarkerSeek score 68.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1260
variable_sites: 32
pi: 0.0117
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CGGAATAAGTAGAAGCCCGA	GGTGGAAACTGATTTGGCTA	50.0	45.0	56.8	55.6	1223-1536 bp	1.0	57.4
ndhF-rpl32_p2	TCGGAATAAGTAGAAGCCCG	GGTGGAAACTGATTTGGCTA	50.0	45.0	56.8	55.6	1224-1537 bp	1.0	57.4
ndhF-rpl32_p3	GAAGCCCGAATCCTATTGACA	GGTGGAAACTGATTTGGCTA	47.6	45.0	57.8	55.6	1212-1525 bp	1.0	57.3
ndhF-rpl32_p4	GTCGGAATAAGTAGAAGCCCG	GGTGGAAACTGATTTGGCTA	52.4	45.0	58.3	55.6	1225-1538 bp	1.0	56.7
ndhF-rpl32_p5	TCGGAATAAGTAGAAGCCCGA	GGTGGAAACTGATTTGGCTA	47.6	45.0	58.3	55.6	1224-1537 bp	1.0	56.1