Hotspot

trnI-CAU-ycf2

IRb · 94661-94726 · 66 bp

Back to Phrynium

Pi 0.0439

MarkerSeek score 65.50

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 66
variable_sites: 6
pi: 0.0439
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	CTCAATTCGACGATCCAGGA	50.0	50.0	58.0	57.1	91-139 bp	1.0	76.7
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	TCTCAATTCGACGATCCAGG	50.0	50.0	58.0	57.1	92-140 bp	1.0	76.6
trnI-CAU-ycf2_p3	AGTTGGGCGCTTTAACCATT	CTCAATTCGACGATCCAGGA	45.0	50.0	58.4	57.1	92-140 bp	1.0	73.7
trnI-CAU-ycf2_p4	AGTTGGGCGCTTTAACCATT	TCTCAATTCGACGATCCAGG	45.0	50.0	58.4	57.1	93-141 bp	1.0	73.7
trnI-CAU-ycf2_p5	TTGGGCGCTTTAACCATTCA	CTCAATTCGACGATCCAGGA	45.0	50.0	58.4	57.1	90-138 bp	1.0	73.7