Hotspot

rpl36-infA

LSC · 87290-87397 · 108 bp

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Pi 0.0389
MarkerSeek score 62.90
Primer available yes
Label rpl36-infA

Feature Metadata

label_name
rpl36-infA
feature_type
igs
region
LSC
length_bp
108
variable_sites
10
pi
0.0389
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-infA_p1 CCTACGGATCAGTCGACATT CAACAAAGATTCGAACGATT 50.0 35.0 56.8 52.5 185-187 bp 1.0 45.1
rpl36-infA_p2 GCCTACGGATCAGTCGACAT CAACAAAGATTCGAACGATT 55.0 35.0 59.3 52.5 186-188 bp 1.0 45.1
rpl36-infA_p3 CCTACGGATCAGTCGACATTT CAACAAAGATTCGAACGATT 47.6 35.0 57.6 52.5 185-187 bp 1.0 45.1
rpl36-infA_p4 CCTACGGATCAGTCGACATT CAACAAAGATTCGAACGATTAG 50.0 36.4 56.8 54.0 185-187 bp 1.0 45.1
rpl36-infA_p5 GCCTACGGATCAGTCGACA CAACAAAGATTCGAACGATT 57.9 35.0 58.9 52.5 186-188 bp 1.0 45.1