Hotspot

trnG-GCC-trnfM-CAU

LSC · 41844-42045 · 202 bp

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Pi 0.0119

MarkerSeek score 62.10

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 202
variable_sites: 5
pi: 0.0119
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	247-279 bp	1.0	84.7
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	248-280 bp	1.0	84.7
trnG-GCC-trnfM-CAU_p3	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	288-320 bp	1.0	84.2
trnG-GCC-trnfM-CAU_p4	AGAAGATGCGGGTTCGATTC	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	241-273 bp	1.0	84.2
trnG-GCC-trnfM-CAU_p5	GAGAAGATGCGGGTTCGATT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	242-274 bp	1.0	84.2