Hotspot

petN-psbM

LSC · 33238-34212 · 975 bp

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Pi 0.00401
MarkerSeek score 64.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
975
variable_sites
9
pi
0.00401
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTATGGGGAAGGAGTGGAC TGCTACTGCACTTTTCATTCT 55.0 38.1 58.1 55.8 1018-1079 bp 1.0 58.1
petN-psbM_p2 GTATGGGGAAGGAGTGGACT TGCTACTGCACTTTTCATTCT 55.0 38.1 58.1 55.8 1017-1078 bp 1.0 58.1
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTTTTCATTCT 50.0 38.1 58.2 55.8 1076-1137 bp 1.0 57.8
petN-psbM_p4 TATGGGGAAGGAGTGGACTC TGCTACTGCACTTTTCATTCT 55.0 38.1 57.8 55.8 1016-1077 bp 1.0 57.7
petN-psbM_p5 GGGGAAGGAGTGGACTCTAG TGCTACTGCACTTTTCATTCT 60.0 38.1 58.2 55.8 1013-1074 bp 1.0 57.3