Hotspot

trnS-GCU-trnG-UCC

LSC · 9339-11875 · 2537 bp

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Pi 0.0126

MarkerSeek score 64.80

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 2537
variable_sites: 68
pi: 0.0126
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AACGGAGAGACCCCTTAACT	50.0	50.0	58.0	57.7	1928-2801 bp	1.0	83.5
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	AACGGAGAGACCCCTTAACT	50.0	50.0	58.0	57.7	1927-2800 bp	1.0	83.5
trnS-GCU-trnG-UCC_p3	TCAAGTAATCAGAGCGAGCG	AACGGAGAGACCCCTTAACT	50.0	50.0	57.8	57.7	1999-2872 bp	1.0	81.9
trnS-GCU-trnG-UCC_p4	CGGAGAGAGAGGGATTCGAA	AACGGAGAGACCCCTTAACT	55.0	50.0	58.3	57.7	1981-2854 bp	1.0	81.0
trnS-GCU-trnG-UCC_p5	AGCAATCCGACGCTTTAGTC	AACGGAGAGACCCCTTAACT	50.0	50.0	58.4	57.7	1930-2803 bp	1.0	80.7