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trnK-UUU-rps16

LSC · 4596-5481 · 886 bp

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Pi 0.00768

MarkerSeek score 62.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 886
variable_sites: 15
pi: 0.00768
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCACTTAAAAGCCGAG	TCAACCAACAGGAACCGTTT	50.0	45.0	58.6	57.9	999-1006 bp	1.0	79.6
trnK-UUU-rps16_p2	TAGTCGCACTTAAAAGCCGA	TCAACCAACAGGAACCGTTT	45.0	45.0	57.3	57.9	1000-1007 bp	1.0	78.3
trnK-UUU-rps16_p3	TCGCACTTAAAAGCCGAGTA	TCAACCAACAGGAACCGTTT	45.0	45.0	57.3	57.9	997-1004 bp	1.0	78.3
trnK-UUU-rps16_p4	AGTCGCACTTAAAAGCCGAG	CTCAACCAACAGGAACCGTT	50.0	50.0	58.6	58.3	1000-1007 bp	1.0	78.0
trnK-UUU-rps16_p5	CGCACTTAAAAGCCGAGTAC	TCAACCAACAGGAACCGTTT	50.0	45.0	57.2	57.9	996-1003 bp	1.0	77.9