Hotspot

ycf1

IRb · 109642-110394 · 753 bp

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Pi 0.00266
MarkerSeek score 60.30
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
IRb
length_bp
753
variable_sites
2
pi
0.00266
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 ACAGATGTCGTTTCTAGTCT GGGGTTGGTGTCATGAGTTT 40.0 50.0 53.5 58.0 976 bp 1.0 43.8
ycf1_p2 AACAGATGTCGTTTCTAGTCT GGGGTTGGTGTCATGAGTTT 38.1 50.0 54.4 58.0 977 bp 1.0 43.0
ycf1_p3 ACAGATGTCGTTTCTAGTCT TAGGGAGTGGCCGTATTTCT 40.0 50.0 53.5 57.8 930 bp 1.0 42.5
ycf1_p4 ACAGATGTCGTTTCTAGTCT TTACGAATGGGGTTGGTGTC 40.0 50.0 53.5 57.8 984 bp 1.0 42.4
ycf1_p5 ACAGATGTCGTTTCTAGTCT ATGTAGGGAGTGGCCGTATT 40.0 50.0 53.5 58.2 933 bp 1.0 42.3