Hotspot

rps19

LSC · 84708-84986 · 279 bp

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Pi 0.00358
MarkerSeek score 43.20
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
LSC
length_bp
279
variable_sites
1
pi
0.00358
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 CGCATAACTACGTCCTCGAG AAAAGTTTGGGTAGAGCCGG 55.0 50.0 58.0 58.1 937-938 bp 1.0 79.4
rps19_p2 CGCATAACTACGTCCTCGAG ATTACCTTCTGGGGAGGTCC 55.0 55.0 58.0 58.1 1019-1020 bp 1.0 79.3
rps19_p3 CGCATAACTACGTCCTCGAG TGGGGAGGTCCGTTTGATAT 55.0 50.0 58.0 58.1 1010-1011 bp 1.0 79.3
rps19_p4 CGGCGGAACCCATATTGTAA AAAAGTTTGGGTAGAGCCGG 50.0 50.0 58.0 58.1 848-849 bp 1.0 79.3
rps19_p5 CGGCGGAACCCATATTGTAA ATTACCTTCTGGGGAGGTCC 50.0 55.0 58.0 58.1 930-931 bp 1.0 79.2