Hotspot

ndhH

SSC · 121604-122785 · 1182 bp

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Pi 0.00423
MarkerSeek score 60.70
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
SSC
length_bp
1182
variable_sites
5
pi
0.00423
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 GGAACAAGCATCCATATAATCCC TGCATGGAAGAATGACTCGG 43.5 50.0 57.1 58.0 1307 bp 1.0 48.8
ndhH_p2 GGGAACAAGCATCCATATAATCC TGCATGGAAGAATGACTCGG 43.5 50.0 57.1 58.0 1308 bp 1.0 48.8
ndhH_p3 GGAACAAGCATCCATATAATCCC GCATGGAAGAATGACTCGGA 43.5 50.0 57.1 57.7 1306 bp 1.0 46.5
ndhH_p4 GGGAACAAGCATCCATATAATCC GCATGGAAGAATGACTCGGA 43.5 50.0 57.1 57.7 1307 bp 1.0 46.5
ndhH_p5 TGGGAACAAGCATCCATATA TGCATGGAAGAATGACTCGG 40.0 50.0 53.8 58.0 1309 bp 1.0 46.1