Hotspot

ndhD

SSC · 115412-116914 · 1503 bp

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Pi 0.00133
MarkerSeek score 45.00
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1503
variable_sites
2
pi
0.00133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 CGGCCCTTTTATGTGGATCA GATACCCTGGGACGGATGTA 50.0 55.0 57.9 58.0 2517 bp 1.0 79.7
ndhD_p2 CGGCCCTTTTATGTGGATCA TTTGGCTCTCGCTCATAACC 50.0 50.0 57.9 58.0 2741 bp 1.0 79.5
ndhD_p3 CTAACGAAGCATGGGGATCC GATACCCTGGGACGGATGTA 55.0 55.0 58.1 58.0 2223 bp 1.0 79.2
ndhD_p4 CTAACGAAGCATGGGGATCC TTTGGCTCTCGCTCATAACC 55.0 50.0 58.1 58.0 2447 bp 1.0 79.0
ndhD_p5 CGGCCCTTTTATGTGGATCA TGTGAATCTGCCTGTCCAAC 50.0 50.0 57.9 58.1 2441 bp 1.0 78.9