Hotspot

accD-psaI

LSC · 59787-60404 · 618 bp

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Pi 0.00163
MarkerSeek score 60.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
618
variable_sites
1
pi
0.00163
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CCAGAAGGGCTTATTCGACC CCATTGCAATTGCCGGAAAT 55.0 45.0 58.0 58.0 794-800 bp 1.0 80.1
accD-psaI_p2 CCAGAAGGGCTTATTCGACC AAGAAGCCATTGCAATTGCC 55.0 45.0 58.0 57.9 800-806 bp 1.0 79.5
accD-psaI_p3 CCAGAAGGGCTTATTCGACC GCCGGAAATACTAGGCCTAC 55.0 55.0 58.0 57.5 783-789 bp 1.0 76.7
accD-psaI_p4 TCCAGAAGGGCTTATTCGAC CCATTGCAATTGCCGGAAAT 50.0 45.0 57.0 58.0 795-801 bp 1.0 72.6
accD-psaI_p5 TCCAGAAGGGCTTATTCGAC AAGAAGCCATTGCAATTGCC 50.0 45.0 57.0 57.9 801-807 bp 1.0 72.0