Hotspot

ndhC-trnV-UAC

LSC · 51208-52380 · 1173 bp

Back to Phryma

Pi 0.00258
MarkerSeek score 61.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1173
variable_sites
3
pi
0.00258
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CGGGGCTAAAATCCCAGAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1295-1305 bp 1.0 78.1
ndhC-trnV-UAC_p2 AATCGGGGCTAAAATCCCAG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.6 57.8 1298-1308 bp 1.0 76.3
ndhC-trnV-UAC_p3 TTCTCCGGCCCTTTCCTAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1315-1325 bp 1.0 75.9
ndhC-trnV-UAC_p4 CGGGGCTAAAATCCCAGAAA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1294-1304 bp 1.0 75.4
ndhC-trnV-UAC_p5 CCCTTTCCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.6 57.8 1307-1317 bp 1.0 74.7