Hotspot

rpoB-trnC-GCA

LSC · 27472-28675 · 1204 bp

Back to Phryma

Pi 0.00249
MarkerSeek score 60.20
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1204
variable_sites
3
pi
0.00249
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AGTTCTTCTGCCAAACCCTG CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1346 bp 1.0 79.4
rpoB-trnC-GCA_p2 GTTCTTCTGCCAAACCCTGA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1345 bp 1.0 79.4
rpoB-trnC-GCA_p3 GCCAAACCCTGATCGATGAA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.2 58.1 1337 bp 1.0 77.5
rpoB-trnC-GCA_p4 AGTTCTTCTGCCAAACCCTG AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.0 58.4 1328 bp 1.0 77.2
rpoB-trnC-GCA_p5 GTTCTTCTGCCAAACCCTGA AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.0 58.4 1327 bp 1.0 77.2