Hotspot

trnK-UUU-rps16

LSC · 4513-5418 · 906 bp

Back to Phryma

Pi 0.00221
MarkerSeek score 60.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
906
variable_sites
2
pi
0.00221
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 1028-1029 bp 1.0 65.5
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 1027-1028 bp 1.0 65.5
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC GTGCTCAACCCACAGGAAC 50.0 57.9 58.3 58.7 1042-1043 bp 1.0 64.8
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 1027-1028 bp 1.0 64.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 1026-1027 bp 1.0 64.7