Hotspot

ndhF-rpl32

SSC · 105804-106703 · 900 bp

Back to Phragmites

Pi 0
MarkerSeek score 62.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
900
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 958-989 bp 1.0 43.7
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 959-990 bp 1.0 43.7
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 960-991 bp 1.0 43.7
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 961-992 bp 1.0 43.7
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTTGACATCGAAGTACGT 55.0 35.0 58.1 53.2 962-993 bp 1.0 43.6