Hotspot

clpP-psbB

LSC · 70171-70679 · 509 bp

Back to Phragmites

Pi 0.00394

MarkerSeek score 59.40

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 509
variable_sites: 3
pi: 0.00394
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACCCAAGTCGCTTCTTCATC	ACCCAGAAACAAGAGCTGTG	50.0	50.0	57.9	58.0	668-671 bp	1.0	79.5
clpP-psbB_p2	ACCCAAGTCGCTTCTTCATC	CACCCAGAAACAAGAGCTGT	50.0	50.0	57.9	58.0	669-672 bp	1.0	79.5
clpP-psbB_p3	ACCCAAGTCGCTTCTTCATC	CCCAGAAACAAGAGCTGTGT	50.0	50.0	57.9	58.0	667-670 bp	1.0	79.5
clpP-psbB_p4	ACCCAAGTCGCTTCTTCATC	AGAAACAAGAGCTGTGTGCA	50.0	45.0	57.9	58.0	664-667 bp	1.0	79.3
clpP-psbB_p5	TCAACCCAAGTCGCTTCTTC	CCCAGAAACAAGAGCTGTGT	50.0	50.0	58.1	58.0	670-673 bp	1.0	79.3