Hotspot

trnP-GGG-psaJ

LSC · 65949-66340 · 392 bp

Back to Phragmites

Pi 0.00686

MarkerSeek score 47.00

Primer available yes

Label trnP-GGG-psaJ

Feature Metadata

label_name: trnP-GGG-psaJ
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 4
pi: 0.00686
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-psaJ_p1	AAAACAAACGCGCTACCAAG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.9	57.9	497-500 bp	1.0	79.4
trnP-GGG-psaJ_p2	CAAAACAAACGCGCTACCAA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.2	57.9	498-501 bp	1.0	79.0
trnP-GGG-psaJ_p3	TTGTACCCAAAACAAACGCG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.8	57.9	505-508 bp	1.0	78.8
trnP-GGG-psaJ_p4	CCGCTACTGCCCATAAAGAA	AAACCTGCTAAAGCCCCAAA	50.0	45.0	57.7	57.9	571-574 bp	1.0	77.6
trnP-GGG-psaJ_p5	TACCCAAAACAAACGCGCTA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.4	57.9	502-505 bp	1.0	77.1