Hotspot

ndhC-trnV-UAC

LSC · 52050-52820 · 771 bp

Back to Phragmites

Pi 0.000865

MarkerSeek score 59.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 1
pi: 0.000865
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	896-897 bp	1.0	79.3
ndhC-trnV-UAC_p2	CTAACCGGGGCTAAAAGTCC	AGCCCAATGCAGAAAAATGC	55.0	45.0	58.0	57.9	989-990 bp	1.0	79.1
ndhC-trnV-UAC_p3	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.0	58.3	897-898 bp	1.0	77.5
ndhC-trnV-UAC_p4	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	895-896 bp	1.0	76.9
ndhC-trnV-UAC_p5	TAACCGGGGCTAAAAGTCCT	AGCCCAATGCAGAAAAATGC	50.0	45.0	58.3	57.9	988-989 bp	1.0	76.6