Hotspot

petN-trnC-GCA

LSC · 19268-20168 · 901 bp

Back to Phragmites

Pi 0.00481

MarkerSeek score 58.80

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 6
pi: 0.00481
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	AGGGGACTGCAAATCCTTTA	50.0	45.0	58.2	56.4	974-980 bp	1.0	66.6
petN-trnC-GCA_p2	CCACTCCTCCCCCATACTAC	AGGGGACTGCAAATCCTTTA	60.0	45.0	58.3	56.4	1030-1036 bp	1.0	65.5
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGTGCCG	50.0	55.0	58.2	59.5	951 bp	0.333333	40.3
petN-trnC-GCA_p4	CCACTCCTCCCCCATACTAC	CCAGTTCAAATCTGGTGCCG	60.0	55.0	58.3	59.5	1007 bp	0.333333	39.3
petN-trnC-GCA_p5	TCCACTCCTCCCCCATACTA	CCAGTTCAAATCTGGTGCCG	55.0	55.0	58.4	59.5	1008 bp	0.333333	38.5