Hotspot

rpl22

LSC · 85499-85897 · 399 bp

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Pi 0.0164

MarkerSeek score 49.20

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 399
variable_sites: 12
pi: 0.0164
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CGCCCTGCAATTTGTACTTG	CAGATCGTATGGTCGGTCAC	50.0	55.0	58.0	57.9	1294-1389 bp	0.6	71.7
rpl22_p2	CGCCCTGCAATTTGTACTTG	CGTCGCCGTAAATAGGAACA	50.0	50.0	58.0	58.1	1773-1853 bp	0.6	70.9
rpl22_p3	CGCCCTGCAATTTGTACTTG	GATCCACTTGGCTACATCCG	50.0	55.0	58.0	58.1	1654-1742 bp	0.6	70.7
rpl22_p4	CGCCCTGCAATTTGTACTTG	CAATCCACTGCCTTGATCCA	50.0	50.0	58.0	57.9	1668-1756 bp	0.6	70.6
rpl22_p5	CGCCCTGCAATTTGTACTTG	ATTCATTGGCGGGGGATTTT	50.0	45.0	58.0	58.1	1053-1117 bp	0.4	56.1