Hotspot

trnV-GAC-rps12

IRa · 131470-133705 · 2236 bp

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Pi 0.0247

MarkerSeek score 67.60

Primer available yes

Label trnV-GAC-rps12

Feature Metadata

label_name: trnV-GAC-rps12
feature_type: igs
region: IRa
length_bp: 2236
variable_sites: 105
pi: 0.0247
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rps12_p1	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1973-2429 bp	1.0	84.3
trnV-GAC-rps12_p2	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1977-2433 bp	1.0	84.2
trnV-GAC-rps12_p3	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1972-2428 bp	1.0	84.2
trnV-GAC-rps12_p4	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	55.0	45.0	58.3	57.7	1975-2431 bp	1.0	83.3
trnV-GAC-rps12_p5	GTCAAGGTGACACTCTACCG	TGGCAACTTTACGTAAGGCA	55.0	45.0	57.7	57.7	1958-2414 bp	1.0	82.8