Hotspot

trnL-UAG-ccsA

SSC · 111434-111527 · 94 bp

Back to Phragmipedium

Pi 0.206

MarkerSeek score 77.30

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 94
variable_sites: 36
pi: 0.206
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	CACAATTGAGATGATCGAAAAGGA	50.0	37.5	57.9	57.6	224-369 bp	1.0	55.6
trnL-UAG-ccsA_p2	TAGACACGCTGCTCTTAGGA	TCACAATTGAGATGATCGAAAAGG	50.0	37.5	57.9	57.6	225-370 bp	1.0	55.6
trnL-UAG-ccsA_p3	TAAGCCGCCATGGTGAAATT	CACAATTGAGATGATCGAAAAGGA	45.0	37.5	58.2	57.6	246-391 bp	1.0	55.4
trnL-UAG-ccsA_p4	TAAGCCGCCATGGTGAAATT	TCACAATTGAGATGATCGAAAAGG	45.0	37.5	58.2	57.6	247-392 bp	1.0	55.4
trnL-UAG-ccsA_p5	AAATTGGTAGACACGCTGCT	CACAATTGAGATGATCGAAAAGGA	45.0	37.5	57.8	57.6	231-376 bp	1.0	55.1